Validation of a polymerase chain reaction-oligochromatography test for detection of influenza A (H1N1) 2009 virus.
Identifieur interne : 000291 ( Main/Exploration ); précédent : 000290; suivant : 000292Validation of a polymerase chain reaction-oligochromatography test for detection of influenza A (H1N1) 2009 virus.
Auteurs : Loreto Fuenzalida [Espagne] ; Cristina Prat ; Silvia Blanco ; Albert Bas ; M J Domínguez ; M Ngeles Hernández ; Carlos Rodrigo ; Vicente AusinaSource :
- Diagnostic microbiology and infectious disease [ 1879-0070 ] ; 2012.
Descripteurs français
- KwdFr :
- Adolescent (MeSH), Adulte (MeSH), Adulte d'âge moyen (MeSH), Chromatographie (méthodes), Enfant (MeSH), Enfant d'âge préscolaire (MeSH), Espagne (épidémiologie), Femelle (MeSH), Grippe humaine (diagnostic), Grippe humaine (virologie), Grippe humaine (épidémiologie), Humains (MeSH), Jeune adulte (MeSH), Mâle (MeSH), Nourrisson (MeSH), Nouveau-né (MeSH), Pandémies (MeSH), Partie nasale du pharynx (virologie), RT-PCR (méthodes), Reproductibilité des résultats (MeSH), Réaction de polymérisation en chaine en temps réel (méthodes), Sensibilité et spécificité (MeSH), Sous-type H1N1 du virus de la grippe A (isolement et purification), Sujet âgé (MeSH).
- MESH :
- diagnostic : Grippe humaine.
- isolement et purification : Sous-type H1N1 du virus de la grippe A.
- méthodes : Chromatographie, RT-PCR, Réaction de polymérisation en chaine en temps réel.
- virologie : Grippe humaine, Partie nasale du pharynx.
- épidémiologie : Espagne, Grippe humaine.
- Adolescent, Adulte, Adulte d'âge moyen, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Jeune adulte, Mâle, Nourrisson, Nouveau-né, Pandémies, Reproductibilité des résultats, Sensibilité et spécificité, Sujet âgé.
- Wicri :
- geographic : Espagne.
English descriptors
- KwdEn :
- Adolescent (MeSH), Adult (MeSH), Aged (MeSH), Child (MeSH), Child, Preschool (MeSH), Chromatography (methods), Female (MeSH), Humans (MeSH), Infant (MeSH), Infant, Newborn (MeSH), Influenza A Virus, H1N1 Subtype (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (epidemiology), Influenza, Human (virology), Male (MeSH), Middle Aged (MeSH), Nasopharynx (virology), Pandemics (MeSH), Real-Time Polymerase Chain Reaction (methods), Reproducibility of Results (MeSH), Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity (MeSH), Spain (epidemiology), Young Adult (MeSH).
- MESH :
- geographic , epidemiology : Spain.
- diagnosis : Influenza, Human.
- epidemiology : Influenza, Human.
- isolation & purification : Influenza A Virus, H1N1 Subtype.
- methods : Chromatography, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Influenza, Human, Nasopharynx.
- Adolescent, Adult, Aged, Child, Child, Preschool, Female, Humans, Infant, Infant, Newborn, Male, Middle Aged, Pandemics, Reproducibility of Results, Sensitivity and Specificity, Young Adult.
Abstract
The outbreak of pandemic influenza A (H1N1) 2009 virus caused the first influenza pandemic disease of the 21st century. In August 2010, the pandemic moved into the post-pandemic period. However, localized outbreaks of various magnitudes continued with a higher rate of disease severity. The aim of this study was to assess a new polymerase chain reaction (PCR)-oligochromatographic assay (Speed-Oligo) in the diagnosis of novel influenza A (H1N1) 2009. A total of 405 nasopharyngeal aspirate specimens from 400 pediatric and adults patients with suspected infection of pandemic influenza A (H1N1) 2009 were analyzed. The sensitivity and specificity values of the Speed-Oligo assay in comparison to reverse transcriptase-PCR assay developed by the Centers for Disease Control and Prevention were 86.5% and 92.2%, respectively. The new assay is simple, rapid, and provides a good sensitivity for detection of influenza A (H1N1) 2009. This assay might be a good alternative to real-time PCR assays for laboratories not equipped with real-time PCR instruments.
DOI: 10.1016/j.diagmicrobio.2011.10.007
PubMed: 22137682
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Nourrisson</term><term>Nouveau-né</term><term>Pandémies</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Sujet âgé</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Espagne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The outbreak of pandemic influenza A (H1N1) 2009 virus caused the first influenza pandemic disease of the 21st century. In August 2010, the pandemic moved into the post-pandemic period. However, localized outbreaks of various magnitudes continued with a higher rate of disease severity. The aim of this study was to assess a new polymerase chain reaction (PCR)-oligochromatographic assay (Speed-Oligo) in the diagnosis of novel influenza A (H1N1) 2009. A total of 405 nasopharyngeal aspirate specimens from 400 pediatric and adults patients with suspected infection of pandemic influenza A (H1N1) 2009 were analyzed. The sensitivity and specificity values of the Speed-Oligo assay in comparison to reverse transcriptase-PCR assay developed by the Centers for Disease Control and Prevention were 86.5% and 92.2%, respectively. The new assay is simple, rapid, and provides a good sensitivity for detection of influenza A (H1N1) 2009. This assay might be a good alternative to real-time PCR assays for laboratories not equipped with real-time PCR instruments.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22137682</PMID><DateCompleted><Year>2012</Year><Month>05</Month><Day>09</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0070</ISSN><JournalIssue CitedMedium="Internet"><Volume>72</Volume><Issue>2</Issue><PubDate><Year>2012</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Diagnostic microbiology and infectious disease</Title><ISOAbbreviation>Diagn. Microbiol. Infect. Dis.</ISOAbbreviation></Journal><ArticleTitle>Validation of a polymerase chain reaction-oligochromatography test for detection of influenza A (H1N1) 2009 virus.</ArticleTitle><Pagination><MedlinePgn>144-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.diagmicrobio.2011.10.007</ELocationID><Abstract><AbstractText>The outbreak of pandemic influenza A (H1N1) 2009 virus caused the first influenza pandemic disease of the 21st century. In August 2010, the pandemic moved into the post-pandemic period. However, localized outbreaks of various magnitudes continued with a higher rate of disease severity. The aim of this study was to assess a new polymerase chain reaction (PCR)-oligochromatographic assay (Speed-Oligo) in the diagnosis of novel influenza A (H1N1) 2009. A total of 405 nasopharyngeal aspirate specimens from 400 pediatric and adults patients with suspected infection of pandemic influenza A (H1N1) 2009 were analyzed. The sensitivity and specificity values of the Speed-Oligo assay in comparison to reverse transcriptase-PCR assay developed by the Centers for Disease Control and Prevention were 86.5% and 92.2%, respectively. The new assay is simple, rapid, and provides a good sensitivity for detection of influenza A (H1N1) 2009. This assay might be a good alternative to real-time PCR assays for laboratories not equipped with real-time PCR instruments.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fuenzalida</LastName><ForeName>Loreto</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació en Ciènces de la Salut Germans Trias i Pujol, Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08916 Badalona, Spain. loretoffi@gmail.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Prat</LastName><ForeName>Cristina</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Blanco</LastName><ForeName>Silvia</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Bas</LastName><ForeName>Albert</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Domínguez</LastName><ForeName>M J</ForeName><Initials>MJ</Initials></Author><Author ValidYN="Y"><LastName>Hernández</LastName><ForeName>M Ángeles</ForeName><Initials>MÁ</Initials></Author><Author ValidYN="Y"><LastName>Rodrigo</LastName><ForeName>Carlos</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Ausina</LastName><ForeName>Vicente</ForeName><Initials>V</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D023361">Validation Study</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>12</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Diagn Microbiol Infect Dis</MedlineTA><NlmUniqueID>8305899</NlmUniqueID><ISSNLinking>0732-8893</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002675" MajorTopicYN="N">Child, Preschool</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002845" MajorTopicYN="N">Chromatography</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007223" MajorTopicYN="N">Infant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007231" MajorTopicYN="N">Infant, Newborn</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053118" MajorTopicYN="N">Influenza A Virus, H1N1 Subtype</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009305" MajorTopicYN="N">Nasopharynx</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058873" MajorTopicYN="N">Pandemics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013030" MajorTopicYN="N" Type="Geographic">Spain</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>07</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2011</Year><Month>10</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>10</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>12</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>12</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>5</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22137682</ArticleId><ArticleId IdType="pii">S0732-8893(11)00442-1</ArticleId><ArticleId IdType="doi">10.1016/j.diagmicrobio.2011.10.007</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Catalogne</li></region></list><tree><noCountry><name sortKey="Ausina, Vicente" sort="Ausina, Vicente" uniqKey="Ausina V" first="Vicente" last="Ausina">Vicente Ausina</name><name sortKey="Bas, Albert" sort="Bas, Albert" uniqKey="Bas A" first="Albert" last="Bas">Albert Bas</name><name sortKey="Blanco, Silvia" sort="Blanco, Silvia" uniqKey="Blanco S" first="Silvia" last="Blanco">Silvia Blanco</name><name sortKey="Dominguez, M J" sort="Dominguez, M J" uniqKey="Dominguez M" first="M J" last="Domínguez">M J Domínguez</name><name sortKey="Hernandez, M Ngeles" sort="Hernandez, M Ngeles" uniqKey="Hernandez M" first="M Ngeles" last="Hernández">M Ngeles Hernández</name><name sortKey="Prat, Cristina" sort="Prat, Cristina" uniqKey="Prat C" first="Cristina" last="Prat">Cristina Prat</name><name sortKey="Rodrigo, Carlos" sort="Rodrigo, Carlos" uniqKey="Rodrigo C" first="Carlos" last="Rodrigo">Carlos Rodrigo</name></noCountry><country name="Espagne"><region name="Catalogne"><name sortKey="Fuenzalida, Loreto" sort="Fuenzalida, Loreto" uniqKey="Fuenzalida L" first="Loreto" last="Fuenzalida">Loreto Fuenzalida</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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